## htsjdk.samtools.metrics.StringHeader # MarkDuplicates --INPUT SRX7354175.sorted.bam --OUTPUT SRX7354175.markdup.sorted.bam --METRICS_FILE SRX7354175.markdup.sorted.MarkDuplicates.metrics.txt --REMOVE_DUPLICATES false --ASSUME_SORTED true --TMP_DIR tmp --VALIDATION_STRINGENCY LENIENT --REFERENCE_SEQUENCE Homo_sapiens_assembly38_noALT_noHLA_noDecoy_patched_ERCC92.fasta --MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP 50000 --MAX_FILE_HANDLES_FOR_READ_ENDS_MAP 8000 --SORTING_COLLECTION_SIZE_RATIO 0.25 --TAG_DUPLICATE_SET_MEMBERS false --REMOVE_SEQUENCING_DUPLICATES false --TAGGING_POLICY DontTag --CLEAR_DT true --DUPLEX_UMI false --FLOW_MODE false --FLOW_QUALITY_SUM_STRATEGY false --USE_END_IN_UNPAIRED_READS false --USE_UNPAIRED_CLIPPED_END false --UNPAIRED_END_UNCERTAINTY 0 --FLOW_SKIP_FIRST_N_FLOWS 0 --FLOW_Q_IS_KNOWN_END false --FLOW_EFFECTIVE_QUALITY_THRESHOLD 15 --ADD_PG_TAG_TO_READS true --DUPLICATE_SCORING_STRATEGY SUM_OF_BASE_QUALITIES --PROGRAM_RECORD_ID MarkDuplicates --PROGRAM_GROUP_NAME MarkDuplicates --READ_NAME_REGEX --OPTICAL_DUPLICATE_PIXEL_DISTANCE 100 --MAX_OPTICAL_DUPLICATE_SET_SIZE 300000 --VERBOSITY INFO --QUIET false --COMPRESSION_LEVEL 5 --MAX_RECORDS_IN_RAM 500000 --CREATE_INDEX false --CREATE_MD5_FILE false --help false --version false --showHidden false --USE_JDK_DEFLATER false --USE_JDK_INFLATER false ## htsjdk.samtools.metrics.StringHeader # Started on: Mon Oct 30 10:59:07 GMT 2023 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE Unknown Library 92898 41189898 6193201 1322076 88822 38286788 0 0.929549 2903111 ## HISTOGRAM java.lang.Double BIN CoverageMult all_sets non_optical_sets 1.0 1 516960 516960 2.0 1 311988 311988 3.0 1 234537 234537 4.0 1 179783 179783 5.0 1 142208 142208 6.0 1 114133 114133 7.0 1 94237 94237 8.0 1 80123 80123 9.0 1 70322 70322 10.0 1 63862 63862 11.0 1 58716 58716 12.0 1 54844 54844 13.0 1 51925 51925 14.0 1 49850 49850 15.0 1 47090 47090 16.0 1 45195 45195 17.0 1 43515 43515 18.0 1 41706 41706 19.0 1 39679 39679 20.0 1 37885 37885 21.0 1 36232 36232 22.0 1 34071 34071 23.0 1 32151 32151 24.0 1 30262 30262 25.0 1 28663 28663 26.0 1 26909 26909 27.0 1 24962 24962 28.0 1 23702 23702 29.0 1 22109 22109 30.0 1 20742 20742 31.0 1 19110 19110 32.0 1 18003 18003 33.0 1 17017 17017 34.0 1 15881 15881 35.0 1 15054 15054 36.0 1 14213 14213 37.0 1 13137 13137 38.0 1 12391 12391 39.0 1 11699 11699 40.0 1 11005 11005 41.0 1 10392 10392 42.0 1 9852 9852 43.0 1 9272 9272 44.0 1 8658 8658 45.0 1 8250 8250 46.0 1 7688 7688 47.0 1 7145 7145 48.0 1 6700 6700 49.0 1 6413 6413 50.0 1 5890 5890 51.0 1 5759 5759 52.0 1 5287 5287 53.0 1 4982 4982 54.0 1 4587 4587 55.0 1 4258 4258 56.0 1 3949 3949 57.0 1 3722 3722 58.0 1 3456 3456 59.0 1 3184 3184 60.0 1 2962 2962 61.0 1 2806 2806 62.0 1 2627 2627 63.0 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