## htsjdk.samtools.metrics.StringHeader # MarkDuplicates --INPUT SRX7354145.sorted.bam --OUTPUT SRX7354145.markdup.sorted.bam --METRICS_FILE SRX7354145.markdup.sorted.MarkDuplicates.metrics.txt --REMOVE_DUPLICATES false --ASSUME_SORTED true --TMP_DIR tmp --VALIDATION_STRINGENCY LENIENT --REFERENCE_SEQUENCE Homo_sapiens_assembly38_noALT_noHLA_noDecoy_patched_ERCC92.fasta --MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP 50000 --MAX_FILE_HANDLES_FOR_READ_ENDS_MAP 8000 --SORTING_COLLECTION_SIZE_RATIO 0.25 --TAG_DUPLICATE_SET_MEMBERS false --REMOVE_SEQUENCING_DUPLICATES false --TAGGING_POLICY DontTag --CLEAR_DT true --DUPLEX_UMI false --FLOW_MODE false --FLOW_QUALITY_SUM_STRATEGY false --USE_END_IN_UNPAIRED_READS false --USE_UNPAIRED_CLIPPED_END false --UNPAIRED_END_UNCERTAINTY 0 --FLOW_SKIP_FIRST_N_FLOWS 0 --FLOW_Q_IS_KNOWN_END false --FLOW_EFFECTIVE_QUALITY_THRESHOLD 15 --ADD_PG_TAG_TO_READS true --DUPLICATE_SCORING_STRATEGY SUM_OF_BASE_QUALITIES --PROGRAM_RECORD_ID MarkDuplicates --PROGRAM_GROUP_NAME MarkDuplicates --READ_NAME_REGEX --OPTICAL_DUPLICATE_PIXEL_DISTANCE 100 --MAX_OPTICAL_DUPLICATE_SET_SIZE 300000 --VERBOSITY INFO --QUIET false --COMPRESSION_LEVEL 5 --MAX_RECORDS_IN_RAM 500000 --CREATE_INDEX false --CREATE_MD5_FILE false --help false --version false --showHidden false --USE_JDK_DEFLATER false --USE_JDK_INFLATER false ## htsjdk.samtools.metrics.StringHeader # Started on: Sun Oct 29 01:08:19 GMT 2023 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE Unknown Library 36028 42950386 6559704 2070928 30104 23006317 0 0.535774 23911631 ## HISTOGRAM java.lang.Double BIN CoverageMult all_sets non_optical_sets 1.0 1 14336467 14336467 2.0 1.165926 2949585 2949585 3.0 1.193457 941889 941889 4.0 1.198026 479785 479785 5.0 1.198784 301843 301843 6.0 1.198909 204202 204202 7.0 1.19893 141906 141906 8.0 1.198934 100805 100805 9.0 1.198934 72811 72811 10.0 1.198934 54126 54126 11.0 1.198934 41078 41078 12.0 1.198934 32441 32441 13.0 1.198934 26351 26351 14.0 1.198934 21950 21950 15.0 1.198934 18688 18688 16.0 1.198934 15943 15943 17.0 1.198934 13896 13896 18.0 1.198934 12372 12372 19.0 1.198934 10949 10949 20.0 1.198934 9939 9939 21.0 1.198934 8723 8723 22.0 1.198934 8098 8098 23.0 1.198934 7210 7210 24.0 1.198934 6729 6729 25.0 1.198934 6046 6046 26.0 1.198934 5572 5572 27.0 1.198934 5185 5185 28.0 1.198934 4681 4681 29.0 1.198934 4446 4446 30.0 1.198934 4137 4137 31.0 1.198934 3845 3845 32.0 1.198934 3508 3508 33.0 1.198934 3357 3357 34.0 1.198934 3059 3059 35.0 1.198934 2956 2956 36.0 1.198934 2727 2727 37.0 1.198934 2689 2689 38.0 1.198934 2421 2421 39.0 1.198934 2350 2350 40.0 1.198934 2147 2147 41.0 1.198934 2096 2096 42.0 1.198934 1977 1977 43.0 1.198934 1864 1864 44.0 1.198934 1810 1810 45.0 1.198934 1719 1719 46.0 1.198934 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